Competitive repopulation of retrovirally transduced haemopoietic stem cells

Long-term maintenance of hematopoiesis in irradiated mice by retrovirally transduced peripheral blood stem cells.

Mobilized peripheral blood stem cells (PBSC) are used as a source of hematopoietic stem cells for transplantation and gene therapy. It is still unclear, however, whether the PBSC are fully equivalent to normal bone marrow hematopoietic stem cells and whether they are able to provide long-term function of transgene in reconstituted mice. In the present study, mobilized PBSC from male mice were t...

Unaltered repopulation properties of mouse hematopoietic stem cells transduced with lentiviral vectors.

Recent studies of retroviral-mediated gene transfer have shown that retroviral integrations themselves may trigger nonmalignant clonal expansion of hematopoietic stem cells (HSCs) in transplant recipients. These observations suggested that previous conclusions of HSC dynamics based on gamma-retroviral gene marking should be confirmed with improved vectors having a more limited capacity to trans...

An in vivo competitive repopulation assay for various sources of human hematopoietic stem cells.

The marrow repopulating potential (MRP) of different sources of human hematopoietic stem cells (HSCs) was directly compared using an in vivo assay in which severe combined immunodeficient disease (SCID) mice were implanted with human fetal bones. HSCs from 2 human lymphocyte antigen (HLA)-mismatched donors were injected individually or simultaneously into the fetal bones of a 3rd distinct HLA t...

Expression of the nlsLacz gene in dendritic cells derived from retrovirally transduced peripheral blood CD34+ cells.

BACKGROUND AND OBJECTIVE Gene transfer and expression of exogenous genetic information coding for an immunogenic protein in antigen presenting cells (APCs) can promote an immune response. This was investigated by retroviral transfer of a marker gene into CD34+ derived APCs. DESIGN AND METHODS To achieve long term expression of a specific transgene in APCs, G-CSF mobilized peripheral blood CD3...

Transduced CD40-Activated B Cells Cytotoxic T Cells Using Retrovirally Efficient Generation of Antigen-Specific